Tris(hydroxymethyl)aminomethane

☑ Tris buffer haingo shandiswe zvakanyanya semugadzirisi wemanucleic acids nemaprotein, asiwo ine zvakawanda zvakakosha zvinoshandiswa.Tris yakashandiswa kuprotein crystal kukura pasi pemamiriro akasiyana epH.
☑ Iyo yakaderera ionic simba reTris buffer inogona kushandiswa kugadzira yepakati faibha yelamin muC. elegans.
☑ Tris zvakare chimwe chezvinhu zvikuru zveprotein electrophoresis buffer.
☑Pamusoro pezvo, Tris ndeyepakati kugadzirira kwevano surfactants, vulcanization accelerators uye mimwe mishonga.Tris inoshandiswawo seyero yetitration.
☑ Trimethylaminomethane inoshandiswa zvakanyanya muacute metabolic uye kufema acidemia.Iyo alkaline buffer uye ine yakanaka buffer mhedzisiro pane metabolic acidosis uye enzyme chiitiko chekuita.
☑Tris inowanzo shandiswa sebiological buffer, uye kukosha kwayo pH i6.8, 7.4, 8.0, 8.8.Yayo chimiro formula, pH kukosha inoshanduka zvakanyanya nekushisa.Kazhinji kutaura, iyo pH kukosha inodzikira ne0.03 apo tembiricha inowedzera nedhigirii rimwe.Tris inoshandiswa zvakanyanya mukugadzirira kwebuffer mhinduro mune biochemistry uye molecular biology kuyedza.Semuyenzaniso, zvese TAE netbe mabuffers (anoshandiswa pakunyungudika kweasidhi) anowanzo shandiswa mukuedzwa kwebiochemical anoda Tris.Nekuti ine mapoka emamino, inogona kuita nealdehydes.
☑ Tris inzvimbo isina kusimba, uye PKA yayo i8.1 pakamuri tembiricha (25 ℃).Zvinoenderana neiyo buffer theory, iyo inoshanda buffer huwandu hweTris buffer iri pakati pe7.0 ne9.2.Iyo pH kukosha kweTris base aqueous solution inenge 10.5.Kazhinji, hydrochloric acid inowedzerwa kugadzirisa pH kukosha kune inodiwa kukosha kuti uwane iyo buffer mhinduro neiyi pH kukosha.Panguva imwecheteyo, isu tinofanirwa kutarisisa kune mhedzisiro yekushisa pa pKa yeTris.Nekuti Tris buffer isingaite alkaline mhinduro, DNA ichabviswa mumhinduro yakadai kuti ivandudze kunyungudika kwayo.Vanhu vanowanzo wedzera EDTA muTris hydrochloric acid buffer kugadzira "Te buffer", iyo inoshandiswa kudzikamisa DNA uye kuchengetedza.Kana iyo acid mhinduro yekugadzirisa pH kukosha ikatsiviwa neacetic acid, iyo "Tae buffer" (Tris / acetate / EDTA) inowanikwa, uye "tbe buffer" (Tris / borate / EDTA) inowanikwa nekuitsiva neboric acid. .Aya mabhafa maviri anowanzo shandiswa mune nucleic acid electrophoresis kuyedza.